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Then Streptavidin-HRP is added and binds to the Biotinylated MYL4 antibody
HMG14 present in the sample is added and binds to antibodies coated on the wells
If the sample contains ANGPTL7
Then a biotinylated detection antibody specific for Chicken PLA2G4D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated KCNJ4 antibody
rT3 (Reverse Triiodothyronine) ELISA Kit Packaging:48 wells Then Streptavidin-HRP is added andThe rT3 (Reverse Triiodothyronine) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with rT3. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to rT3. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme
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