Human VIP (Vasoactive Intestinal Peptide) ELISA Kit AP2 the Buffer WBR is washed

the Buffer WBR is washed to remove the residual PCR inhibitor on the purified column

and the final DNA product Eluted with Buffer TE

The concentration of Human NGAL in the samples is then determined by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Human OSM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Only those wells that contain Mouse REN

Human VIP (Vasoactive Intestinal Peptide) ELISA Kit AP2 the Buffer WBR is washedThe Human VIP (Vasoactive Intestinal Peptide) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human VIP. During the reaction, Human VIP in the sample or standard competes with a fixed amount of Human VIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human VIP. Excess conjugate and unbound sample or standard are washed away, and Avidin Horseradish