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Then a biotinylated detection antibody specific for Rat LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
MMP19 present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Human KCNE1
And then biotinylated Mouse CHIT1 Antibody is added and binds to CHIT1 in the sample
C6 present in the sample is added and binds to antibodies coated on the wells
Human TFEB (Transcription factor EB) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human TFEB (Transcription factor EB) ELISA Kit measures TFEB in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TFEB antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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