Human DA (Dopamine) ELISA Kit Podocalyxin-like protein 1 only those wells that contain

only those wells that contain Rat TNF-alpha

TGFBR1 present in the sample is added and binds to antibodies coated on the wells

Then a biotinylated detection antibody specific for Human GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LNGFR

and samples are incubated for 45 minutes at room temperature (RT)

Human DA (Dopamine) ELISA Kit Podocalyxin-like protein 1 only those wells that containThe Human (DA) Dopamine ELISA Kit measures Dopamine in samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and color develops. The