Human DEFB3 (Beta-defensin 3) ELISA Kit CLG1 8-NitroG in the sample or

8-NitroG in the sample or standard competes with a fixed amount of biotin-labeled 8-NitroG for sites on a pre-coated antibody specific to 8-NitroG

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIRT1

The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-33

Substrate solution is then added and color develops in proportion to the amount of Mouse OSTN

Then Streptavidin-HRP is added and binds to the Biotinylated ADAM7 antibody

Human DEFB3 (Beta-defensin 3) ELISA Kit CLG1 8-NitroG in the sample orThe Human DEFB3 (Beta defensin 3) ELISA Kit measures DEFB3 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti DEFB3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection