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it binds with GAL7 bound to precoated antibody
Then Streptavidin-HRP is added and binds to the Biotinylated ANXA3 antibody
The concentration of Mouse PMP22 in the samples is then determined by comparing the OD of the samples to the standard curve
Only those wells that contain Bovine PGF
Then a biotinylated detection antibody specific for Porcine Ank-B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat MT (Melatonin) ELISA Kit Packaging:48 wells it binds with GAL7 boundThe Rat MT (Melatonin) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Rat MT. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Rat MT. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme substrate
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