Human ETFDH (Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial) ELISA Kit Packaging:96 wells Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Rat MANF

Human Cys-C in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human Cys-C

Then a biotinylated detection antibody specific for Human NAGase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

AHRR present in the sample is added and binds to antibodies coated on the wells

Then a biotinylated detection antibody specific for Human PR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Human ETFDH (Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human ETFDH (Electron transfer flavoprotein ubiquinone oxidoreductase, mitochondrial) ELISA Kit measures ETFDH in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ETFDH antibody. Add standard and properly diluted sample into relevant well respectively. After incubation,