EnTurbo SYBR Color qPCR SuperMix (High ROX Premixed) ELISA then added a HRP-conjugated antibody

then added a HRP-conjugated antibody specific to Mouse E2 to each microplate well and incubated

The concentration of Human VASP in the samples is then determined by comparing the OD of the samples to the standard curve

Recombinant Human Inter-alpha-trypsin Inhibitor Heavy Chain H3 is produced by our Mammalian expression system and the target gene encoding Leu35-Asp651 is expressed with a 6His tag at the C-terminus

overnight digestion

only those wells that contain Rat ADP and HRP-conjugated antibody will exhibit a change in color

EnTurbo SYBR Color qPCR SuperMix (High ROX Premixed) ELISA then added a HRP-conjugated antibodyEnTurbo+ SYBR Color qPCR SuperMix is an optimized 2 x real time PCR master mix containing HotStar aq DNA Polymerase, SYBR Green I fluorochrome dye, dNTP and Mg2+ and High ROX. In addition, the balanced K+ and NH4+ ion ratios in the buffer promote specific primer annealing. To ensure a highly sensitive and specific PCR reaction, the reaction can be initiated by simply adding the primer and cDNA template to the ready to use PCR master mix. The unique