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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TGFbI
The Mouse MUC2 (Mucin 2) ELISA Kit is a Sandwich enzyme immunoassay
Then a biotinylated detection antibody specific for Mouse MIP-1Beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Mouse Irisin in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human EMMPRIN/CD147 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human ACY1 (Aminoacylase-1) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Human (ACY1) Aminoacylase 1 ELISA Kit measures Aminoacylase 1 in samples. The plate has been pre coated with Human ACY1 antibody. ACY1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ACY1 Antibody is added and binds to ACY1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ACY1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
