Human GPC4 (Glypican-4) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added and

Then Streptavidin-HRP is added and binds to the Biotinylated U2 antibody

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFb1

Then a biotinylated detection antibody specific for Mouse CTXI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Calculate the concentration of Gfpt1 in the sample by plotting standard curve

The Mouse IL-5 ELISA kit is a sandwich enzyme immunoassay

Human GPC4 (Glypican-4) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Human GPC4 (Glypican 4) ELISA Kit measures GPC4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti GPC4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.