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The concentration of DLL4 in the sample was calculated by drawing a standard curve
And then biotinylated Chicken cath-K Antibody is added and binds to cath-K in the sample
Calculate the concentration of IL-6Rα in the sample by plotting standard curve
a horseradish peroxidase conjugated mouse anti-Human EGFR Antibody is added to the wells and binds to EGFR in the sample
Substrate solution is then added and color develops in proportion to the amount of Human AKR1C4
Rat STC1 (Stanniocalcin-1) ELISA Kit Packaging:96 wells The concentration of DLL4 inThe Rat STC1 (Stanniocalcin 1) ELISA Kit measures STC1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Stc1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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