Mouse EGF (Epidermal growth factor) ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCFR

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TF

Then a biotinylated detection antibody specific for Rat C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNa

The plate has been pre-coated with Human FZD9 antibody

Mouse EGF (Epidermal growth factor) ELISA Kit Packaging:96 wells Standards or samples are addedThe Mouse EGF (Epidermal growth factor) ELISA Kit measures EGF in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti EGF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection