APOF (Apolipoprotein F) ELISA Kit (Small Sample) Packaging:96 wells The reverse transcription primer provided

The reverse transcription primer provided along with EntiLink 1st Strand cDNA Synthesis Kit is the mixture of pd(N)9 and oligo dT(18)

and the Human NGAL standard plate wells that pre-coated using protein-related techniques are provided separately

Then a biotinylated detection antibody specific for Human MFGE8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The concentration of Simian IL4 in the samples is then determined by comparing the OD of the samples to the standard curve

The OD value is proportional to the concentration of Rat FN

APOF (Apolipoprotein F) ELISA Kit (Small Sample) Packaging:96 wells The reverse transcription primer providedThe APOF (Apolipoprotein F) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human APOF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human APOF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,