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Bovine Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol
The OD value is proportional to the concentration of Mouse LTF
The concentration of GADD45A in the sample was calculated by drawing a standard curve
a horseradish peroxidase conjugated mouse anti-Human B4GALT1 Antibody is added to the wells and binds to B4GALT1 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated CLPS antibody
Human PCNA (ProlifeRating Cell Nuclear Antigen) ELISA Kit Packaging:96 wells Bovine Cortisol in samples (orThe Human PCNA (ProlifeRating Cell Nuclear Antigen) ELISA Kit measures PCNA in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PCNA antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
