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Substrate solution is then added and color develops in proportion to the amount of Human HGS
Then Streptavidin-HRP is added and binds to the Biotinylated SFTPD antibody
Then a biotinylated detection antibody specific for Human OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Mouse CTSB Antibody is added and binds to CTSB in the sample
it binds with BCL2L14 bound to precoated antibody
Human GRN (Granulins) ELISA Kit AN Substrate solution is then addedThe Human GRN (Granulins) ELISA Kit measures GRN in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti GRN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then,
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